Biolistic inoculation of plants with Tomato yellow leaf curl virus DNA
نویسندگان
چکیده
منابع مشابه
Biolistic inoculation of plants with tomato yellow leaf curl virus DNA.
Tomato yellow leaf curl virus (TYLCV) full-length DNA was amplified by PCR and cloned into a bacterial plasmid. The cloned TYLCV DNA was excised from the plasmid, ligated and the resulting monomeric circular double-stranded TYLCV DNA was used to inoculate tomato (Solanum lycopersicom) and datura (Datura stramonium) plants by particle bombardment. The bombarded plants produced typical disease sy...
متن کاملNatural recombination between Tomato yellow leaf curl virus-is and Tomato leaf curl virus.
The complete genome sequences (2791 and 2793 nt) of isolates of Tomato yellow leaf curl virus-Is (TYLCV-Is) from Spain (SP72/97) and Portugal (Port2/95) were determined. These isolates are closely related to TYLCV-Is isolates reported in Japan (Japan-A and Japan-S) and Israel (Israel/Mild). Comparison of all sequenced isolates of TYLCV-Is showed that part of the genome comprising the intergenic...
متن کاملPhenotypic and Molecular Screening of Tomato Germplasm for Resistance to Tomato Yellow Leaf Curl Virus
Tomato yellow leaf curl virus (TYLCV) is a major tomato virus in tropical and subtropical regions. In this study, 134 accessions of Solanum lycopersicum and six accessions of Solanum peruvianum were assessed for resistance to an Iranian isolate of TYLCV. Plants were inoculated using whiteflies (Bemisia tabaci) and the reaction of plants was evaluated based on either disease symptoms or viral DN...
متن کاملComplete Genomic Sequence of a Strain of Tomato Yellow Leaf Curl Virus from Iran
Background and Aims: Tomato yellow leaf curl virus (TYLCV) is one of the most destructive viruses of tomato that leads to reduced tomato yield up to 100% in tropical and subtropical regions. In this study, the complete sequence of TYLCV isolate from Hormozgan province, Iran and its recombination evsent was determined. Methods: TYLCV infected tomato was collected from Hormozgan province. Total D...
متن کاملA dramatic physiological and anatomical changes of tomato plants infecting with tomato yellow leaf curl germinivirus
This study was to investigate the changes of physiological and anatomical in tomato plants infected with tomato yellow leaf curl virus (TYLCV) isolate. it was found the growth characteristic, cell membrane stability index, photosynthetic pigment, carbohydrate contents and related to mineral ions contents of tomato plants. As were decreased, when compared with healthy plants. While electrical le...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2007
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2007.04.011